Figure 5.

Temporal expression of Pax2 and Pax6 genes during development of Branchiostoma lanceolatum, B. floridae, Oryzias latipes, and Danio rerio embryogenesis. cDNA sequences were amplified across the paired encoding exons, as indicated by arrows. (A) For B. lanceolatum and B. floridae, single isoforms were detected for Pax258 and Pax46 genes. (B) For O. latipes Pax2.1, an isoform containing exon 2a was detectable from neurula stage. Pax2.1(del35) and Pax2.2(ext24+) as well as Pax6.1(5a) isoforms are expressed across all developmental stages. Pax6.3 gene does not possess exon 5a. (C) No alternatively spliced isoforms were detected in the case of D. rerio Pax2.1 and Pax2.2 genes. Isoforms including exon 5a are present for both Pax6.1a and Pax6.1b genes.