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Decrease in the stability of claudin-2 mRNA by quercetin. A549 cells were treated with 10 μM actinomycin D in the absence and presence of 50 μM quercetin for the periods indicated. After isolation of total RNA, semi-quantitative RT-PCR (upper images) and quantitative real time PCR (lower graph) were performed using specific primers for claudin-2. n = 3. ** p < 0.01 is significantly different from control.
