Effects of sesamin on insulin content, GTT, IRT and ITT in mice. C57BL/6J mice were injected intraperitoneally with BSA (120 mg/kg, as a control), AGEs (120 mg/kg) or AGEs plus sesamin (160 mg/kg) for 4 weeks. (A) Representative photomicrographs of immunofluorescent staining for insulin in pancreatic islets. Scale bar = 50 μm. (B) Histogram represents quantitative analysis of insulin content in each experimental group. GTT (C) and IRT (D) were performed after intraperitoneal injection of glucose (1.5 mg/g). Values are expressed as mean ± SD, n = 6 per group. *
p < 0.05 versus BSA group. #
p < 0.05 versus AGEs group. MFI: Mean fluorescence intensity; GTT: Glucose tolerance test; IRT: Insulin releasing test.