Figure 5. MoPRX1 detoxifies host ROS and is involved in rice root infection.
(a) Sheath assays with compatible Oryza sativa L. cv. Nakdongbyeo showed delayed and restricted growth of ΔMoPrx1 in rice cells. Sheaths were observed at 24 and 48 hpi inoculated with 2 × 104 conidia/ml. (b) ROS detection by DAB (3,3´-diaminobenzidine) staining, and (c) H2DCFDA (5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester) staining in inoculated rice sheaths at 48 hpi. DIC images were captured using a 20-ms exposure to transmitted light with a DIC filter. Fluorescence images were captured using a 200-ms exposure to absorbed light using a GFP filter. Bar = 50 μm. (d) Root infection from rice seedlings (Oryza sativa L. cv. Nakdongbyeo) by wild-type, ΔMoprx1 and MoPRX1c strains. White arrow indicates the infection site on the root surface.