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. 2015 Jul 2;5:11816. doi: 10.1038/srep11816

Figure 3. Apoptosis induced by infection of SFTSV in HepG2 cells.

Figure 3

(A) Cells were infected with SFTSV at an m.o.i of 1. In situ apoptosis was detected from 24–72 hrs p.i. by an FITC-dUTP labeled TUNEL assay. (B) Transcript levels of TNF-α, FasL, and TRAIL from 6–72 hrs p.i. in infected cells were measured by realtime RT-PCR with specific primers to corresponding cytokines. (C) Concentration of TNF-α induced in infected cells. Cultural media of cells infected with SFTSV were collected at various times points p.i. and used for ELISA measurement of the concentration of TNF-α. The assays were repeated at least three times.