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. 2015 Jul 2;5:11816. doi: 10.1038/srep11816

Figure 8. Regulation of proinflammatory cytokine gene transcripts by NFκB.

Figure 8

HepG2 cells were pretreated with an NFκB specific inhibitor at 100 nM 1.5 hrs prior to SFTSV infection. Total RNA were prepared at 24, 48, and 72 hrs p.i for reverse transcription. cDNA was used for realtime PCR with specific primers to measure fold changes of cytokine transcripts at different time points. Each assay was repeated at least three times. (A-F) Fold change of IL-6, CCL5, IP-10, MIP-3a, IL-8, and IL-1β transcripts. (G) Inhibition of viral RNA replication by the inhibitor of NFκB. Realtime RT-PCR with specific primers to the viral S gene was performed to measure the S gene copy numbers in infected cells treated or non-treated with the NFκB inhibitor. Mean fold changes plus standard deviation of the viral S copy numbers were presented from two or three independent assays (*p < 0.05).