FIG 5 .

Reduction in cellular membrane cholesterol does not affect viral attachment to cells. (A) Schematic of the virus attachment assay. (B) U2OS cells were treated with the S1P inhibitor (25 µM) or the 1% DMSO vehicle for 24 h and then with 62.5 µM cholesterol for the indicated amounts of time. To assess viral attachment, cells were exposed to fluorescein-labeled rVSV-ANDV GP at 4°C (1.5 IU per cell). Bound virus was quantified by flow cytometry. rVSV-ANDV GP binding and infection were performed in parallel, and values are normalized to binding and infection, respectively, in vehicle-treated cells (n = 4; two independent experiments).