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. 2015 Jul 7;6(4):e00959-15. doi: 10.1128/mBio.00959-15

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Copyright © 2015 Laor et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 5 — Gaf1 localization in the nucleus is controlled by TORC1. (A to D) TORC1 negatively regulates the translocation of Gaf1 from the cytoplasm into the nucleus. Wild-type, tor2-51, or mip1-310 cells carrying Gaf1-GFP were grown to mid-log phase in minimal medium (EMM) at 25°C (0 h) and then shifted to 32°C for 4 h to inactivate Tor2 or Mip1. Cells were scored for intracellular Gaf1-GFP localization as for Fig. 4C. (E and F) TORC2-Gad8 has a minor effect on Gaf1 localization. Wild-type, Δtor1, or Δgad8 cells carrying Gaf1-GFP were grown in minimal medium and shifted to proline for 1 h (Pro^1h). Cells were scored for intracellular Gaf1-GFP localization as described above.