Table 2. T. urartu gliadin genes and their corresponding protein spots as identified using 2-DE and MALDI-TOF/TOF-MS.
Spot | %Vol a | Gene | MW(kD) | pI |
---|---|---|---|---|
1 | 1.98 | Gli-α-2 | 32.11 | 8.27 |
Gli-α-3 | 31.63 | 8.27 | ||
2 | 5.09 | Gli-α-4 | 34.75 | 8.49 |
3 | 4.71 | Gli-α-5 | 32.79 | 7.71 |
Gli-α-6 | 33.53 | 7.71 | ||
Gli-α-7 | 33.92 | 7.71 | ||
Gli-α-12 | 33.80 | 7.72 | ||
4 | 12.00 | Gli-α-8 | 32.62 | 7.31 |
5 | 2.21 | Gli-α-9 | 33.06 | 6.69 |
6 | 18.79 | Gli-α-10 | 34.94 | 8.02 |
Gli-α-11 | 34.70 | 8.02 | ||
7 | 1.84 | Gli-α-13 | 33.05 | 6.90 |
8 | 26.80 | Gli-γ-1 | 32.67 | 8.33 |
9 | 10.86 | Gli-γ-1 | 32.67 | 8.33 |
10 | 11.61 | Gli-γ-1 | 32.67 | 8.33 |
11 | 3.54 | Gli-γ-2 | 31.81 | 8.33 |
12 | 0.58 | Gli-γ-3 | 39.12 | 7.60 |
aPercentage of the total spot volume of the extracted gliadin fraction that is accounted for by the indicated spot.