Fig. 2.

Rootletin knock-down does not affect Ch lineage cell divisions or cilium formation. a, b Stage 17 embryos stained with anti-CPO (green) and anti-Futsch (magenta). a Wild type, showing lch5 and v’ch1 Ch neurons. Three cell types found in the chordotonal organ are indicated (neuron, scolopale, cap). Also indicated are ES neurons (lesA–C, v’es2). b Rootletin knock-down, all these cell types are visible. c, d Stage 16 embryos stained with anti-Futsch (magenta) and anti-HRP (green). c Wild type, showing the HRP-positive cilia at the tips of the Ch neurons (lch5 and vchAB). d Rootletin knock-down, showing that the ch neuron cilia are present. e, f Cilia of adult femoral Ch neurons detected with mCD8-GFP. The terminal dendrite of single neurons is shown with the non-ciliary inner segment (is) and cilium (ci, yellow line) indicated. e Wild type. Cilium length = 9.5 μm (f) Rootletin knock-down., Cilium length = 9.8 μm