Fig. 3.

Rootletin knock-down results in loss of rootlet and proximal centriole. Transmission electron microscopy of Ch neuron dendrites in the adult antenna (Johnston’s organ). a–c Wild type. a Longitudinal section of cilium base showing the proximal centriole (black arrow, pc) and a robust ciliary rootlet structure (black arrow, cr). b Longitudinal section of cilium showing the axoneme (ax), distal centriole (dc), proximal centriole (pc), and ciliary rootlet (cr). c Transverse section of cilium at a level proximal to the ciliary dilation. Regular axonemal ninefold symmetry is visible. d–f Rootletin knock-down. d, e Two examples of longitudinal sections labelled as before. Note the lack of ciliary rootlet structure and the proximal centriole. Instead, electron-dense aggregates are visible, possibly representing remains of the ciliary rootlet (*cr). f Transverse section at a level proximally to the ciliary dilation. Regular axonemal ninefold symmetry is apparent