Table 3.

List of PCR primers used in this study

Primer name	Sequence (5′ to 3′)	Target
Umps_F	CCACAAAACCCATTGCCTCAACA	5′-end of UMPS
Umps_R	TCCGTGCTGTCTTCCAGGTCTT	3′-end of UMPS
Umps_cDNAF	CCAAGCTTTGCATGGCATTCAACAAGGTGAA	5′-end of UMPS cDNA
Umps_cDNAR	GGAATTCCCAACCTCATTTGAGAGGCAAT	3′-end of UMPS cDNA
Umps_CDRF	CACCCATGGCAACTTCAACTCCCTCAGT	5′-end of UMPS CDR
Umps_CDRR	CACAAGCTTCATGCCAGCGTGGCCTCATA	3′-end of UMPS CDR
Ptub_F	CCAAGCTTTGCATGGCATTCAACAAGGTGAA	TUBULIN1 promoter
Ptub_R_Umps5′	GAGTTGAAGTTGCCATCTTGAACGACTTCCCTCGACAA	5′-end of UMPS CDR and 3′-end of TUBULIN promoter
Umps3′_Tact_F	GCCACGCTGGCATGAGGTGAGCCTGAAACAGCTTTCTG	3′-end of UMPS CDR and 5′-end of ACT1 terminator
Tact_R	TCGGTACCGGTTGTTGAGTTGAGCACTGGAC	3′-end of ACT1 terminator
Tact_R2	CCCCAGCTGCTACTGCTATC	A middle part of ACT1 terminator
Prbc_F	CTCAATCGGCCGATTTCATGCATGA	5′-end of RBCS promoter
Trbc_R	GTGCTGGTCTGTTTCCATGCAGTCAT	3′-end of RBCS terminator
Ptub3′_Umps_CDRF	GGGAAGTCGTTCAAGATGGCAACTTCAACTCCCTCAG	3′-end of TUBULIN promoter and 5′-end of UMPS CDR
Umps_CDRR_Tact5′	TGTTTCAGGCTCACCTCATGCCAGCGTGGCCTCATA	5′-end of ACT1 terminator and 3′-end of UMPS CDR
G418F	GATCGGCCATTGAACAAGAT	nptII
G418R	GCGATACCGTAAAGCACGAG	nptII
Umps_QRT_F	TCTTCGACAAGTGGGATGATGG	Position 908–930 of UMPS CDR
Umps_QRT_R	TGTTACCGATGTCCGCAAAC	Position 993–1012 of UMPS CDR
18SrRNA_QRT_F	GGATCAATTGGAGGGCAAGT	Position 627–646 of 18S rRNA
18SrRNA_QRT_R	GCCCGAAATCCAACTACGAG	Position 713–732 of 18S rRNA