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. 2015 Jul 2;6:468. doi: 10.3389/fpls.2015.00468

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Copyright © 2015 Toscano-Morales, Xoconostle-Cázares, Cabrera-Ponce, Hinojosa-Moya, Ruiz-Salas, Galván-Gordillo, Guevara-González and Ruiz-Medrano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes of heterozygous (±) insertional mutants (SALK_045146) correlate with AtTCTP2 expression levels. (A) qRT-PCR was performed to determine transcript levels of AtTCTP2, AtTCTP1, and 18S rRNA (as endogenous control) using total RNA extracted from several AtTCTP2 mutants and comparing their expression against WT lines. (B) Severity of phenotype of heterozygous (±) AtTCTP2 mutants ordered by area/size correlates with AtTCTP2 expression levels, and are not associated to a decrease in AtTCTP1 transcript levels. Both qRT-PCR and analysis of phenotype were performed in plants 25 days after germination. Size bars = 2 cm.