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. 2015 Jul 2;6:468. doi: 10.3389/fpls.2015.00468

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Copyright © 2015 Toscano-Morales, Xoconostle-Cázares, Cabrera-Ponce, Hinojosa-Moya, Ruiz-Salas, Galván-Gordillo, Guevara-González and Ruiz-Medrano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of AtTCTP2 and AtTCTP1 in regenerated tobacco plants. Tissue from regenerated plants harboring both unmodified and modified versions of AtTCTP2 and AtTCTP1 were analyzed by confocal laser-scanning microscopy. (A) AtTCTP2-GFP accumulates in stomata (insert: bottom-left) and nuclei (N, arrowheads) in leaves; (B) while in roots its signal was associated to nuclei and vascular tissue (N, arrowheads). (C) AtTCTP1-GFP also accumulates in stomata (insert: bottom-left) and nuclei in leaves, but not in (D) root nuclei. (E–F) Representative wild-type controls for leaf and root are shown. Bars = 25 μm.