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Cyclopamine pre-treatment attenuates seizure-induced glial proliferation. Quantification of immunofluorescence staining for total PCNA+ cells A, Iba1/PCNA double-positive cells B, GFAP/PCNA double-positive cells C, and Olig2/PCNA double-positive cells D. Scale bars for A are 50μm. *, **, *** and **** represent significance determined by unpaired t-test analysis for p<0.05, p<0.01, p<0.001 and p<0.0001.
