FIGURE 5.

DCs isolated from SPC^Cre/CARMA3^F/F mice show impaired maturation, migration, and Ag processing. SPC^Cre/CARMA3^+/+ and SPC^Cre/CARMA3^F/F mice received one OVA/alum immunization on day 0, followed by 40 μg DQ-OVA i.t. on day 7. Twenty-four hours after DQ-OVA administration the (A) lungs and (B) thoracic lymph nodes were isolated and the number of mDCs, CD80⁺ mDCs, CD86⁺ mDCs, OX40L⁺ mDCs, CCR7⁺ mDCs, and DQ-OVA⁺ mDCs were determined by flow cytometry. Data are means ± SEM of eight mice per group from two experiments. *p < 0.05.