Table 1. Listed here are tomogram sizes for which binning will be applied to tomogram, reference and mask.

Volume size	Automatic binning factor
≤256 MB	0×
256–512 MB	1×
512 MB–1 GB	2×

Using binning ensures that the machine will never exceed the available memory during processing. However, users should truncate their tomogram to the interesting region and reduce memory consumption rather than forcing the server to bin all files and proceed at a lower resolution than necessary.