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. 2015 Apr 16;43(Web Server issue):W109–W116. doi: 10.1093/nar/gkv345

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Tissue and cell-specific expression patterns of one novel intergenic locus are shown. (A) Structure of the novel intergenic locus (blue boxes correspond to introns), TCONS_00962903, in the human genome (release hg19), is displayed in the ‘RGV JBrowse session’. Four RNA-seq data sets were selected to illustrate the transcript abundance of this promising candidate in human testes (Chalmel, F. and Rolland, A.D., in preparation) (36) as well as in rodent meiotic and post-meiotic germ cells (32,33). The amount of transcript determined in each tissue/cell and in each study is displayed as color-coded red heat maps. Red histogram bars represent the sequence conservation score distributions between 100 species as provided by the UCSC genome browser (phastCons scores, y-axis ranges from 0 to 1). TCONS_00962903 detection at the RNA level was further confirmed by RT-PCR in four rat (B) and mouse (C) tissue samples, including total testis (TT), brain (BR), kidney (KI), liver (LI) and lung (LU). RT-PCR analysis was also performed in four human tissue samples (D), including total testis (TT), epididymis (EP), seminal vesicle (SV) and prostate (PR), as well as five isolated testicular cell populations (E) including Leydig cells (LC), peritubular myoid cells (PC), Sertoli cells (SC), spermatocytes (Spc), round spermatids (rSpt) and total testis (TT) as positive control.