TABLE. Molecular methods for the detection of Strongyloides stercoralis.
cPCR n (%)a | qPCR n (%)b | |||||
---|---|---|---|---|---|---|
Samples | n | Positive | Negative | Positive | Negative | |
Group I | 30 | 23 (76.7) | 7 (23.3) | 27 (90) | 3 (10) | |
(27.83-39.2)c | NA | |||||
Group II | 40 | 7 (17.5) | 33 (82.5) | 7 (17.5) | 33 (82.5) | |
(33.13-38.39)c | (40.41-46.89)c | |||||
Group III | 30 | 4 (16.7) | 36 (83.3) | 3 (10) | 27 (90) | |
(30.7-37.08)c | (39.64-44.73)c |
: kappa coefficient (ҡ) = 0.710 (0.565-0.855)
: ҡ = 0.587 (0.417-0.757)
: threshold cycle value range; cPCR: conventional polymerase chain reaction; NA: no amplification; qPCR: quantitative PCR.