Fig. 2: trees showing bias due to including genotypic data of hybrid strains. The left tree correspond to a neighbour-joining tree based on a simulated multilocus enzyme electrophoresis dataset. This dataset was based on multilocus sequence typing allelic profiles of 13 loci corresponding to discrete typing units TcI (green boxes), TcII (yellow boxes), TcIII (blue boxes) and TcIV (red boxes). The right tree shows a biased topology due to including of hypothetical hybrid profiles resulting of TcII and TcIII hybridisation. It can be observed that TcIII does not cluster with TcI and TcIV as in the left tree.