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. 2015 Jul 1;10(7):e0131767. doi: 10.1371/journal.pone.0131767

Fig 3. MyD88, IRAK1 and TRAF6 regulate Rv3416 and Nef mediated inhibition of apoptosis in macrophages.

Fig 3

For Panel A, PMA stimulated THP1 cells were transfected with control siRNAs (thin lines) or specific siRNAs to indicated molecules (thick lines) for 36h followed by stimulations with 1 μg/ml Pam3CSK4 (Pam) along with 20 μg/ml Rv3416 and 15 μg/ml Nef for 24h. Cells were stained with Annexin V-APC. Data from one of three independent experiments are shown. Bar graphs adjacent to histograms in Panel A show relative MFIs of the histograms. For Panel B, cytoplasmic extracts from cells cultured as described above were probed for indicated molecules and analyzed by western blots. MOCK represents cells transfected with control siRNAs. Numbers below the blots indicate the relative intensities of the bands. Data from one of three experiments are shown. In Panel A, P<0.04 Control siRNA+Pam+Rv3416+Nef vs siTRAF6+Pam+Rv3416+Nef.