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. 2015 Jul 1;10(7):e0131767. doi: 10.1371/journal.pone.0131767

Fig 5. Molecular sensors of calcium influx regulate inhibition of apoptosis by Rv3416 and Nef in macrophages.

Fig 5

For Panel A, PMA stimulated THP1 cells were transfected with control siRNAs (thin lines) or specific siRNAs to indicated molecules (thick lines) for 36h followed by stimulations with 1 μg/ml Pam3CSK4 (Pam) along with 20 μg/ml Rv3416 and 15 μg/ml Nef for 24h. Cells were stained with Annexin V-APC. Bar graphs adjacent to histograms in Panel A show relative MFIs of the histograms. Data from one of three independent experiments are shown. For Panel B, PMA stimulated THP1 cells were transfected with siRNAs to indicated molecules for 36h followed by stimulations with 1 μg/ml Pam3CSK4 along with 20 μg/ml Rv3416 or 15 μg/ml Nef or both for 24h. Cytoplasmic extracts were probed for indicated molecules and analyzed by western blots. MOCK represents cells transfected with control siRNAs. Numbers below the blots indicate the relative intensities of the bands. Data from one of three experiments are shown. In Panel A, P<0.009 for Pam+Rv3416+Nef vs Pam+Rv3416+Nef+siSTIM1; P<0.02 for Pam+Rv3416+Nef vs Pam+Rv3416+Nef+siSTIM2; P<0.01 for Pam+Rv3416+Nef vs Pam+Rv3416+Nef+siORAI1.