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. 2015 Jul 1;10(7):e0131767. doi: 10.1371/journal.pone.0131767

Fig 7. Nef synergizes with live M. tuberculosis infection to inhibit apoptosis in macrophages.

Fig 7

PMA stimulated THP1 cells were stimulated with 1 μg/ml Pam3CSK4 (Pam) and infected with 2 MOI of M. tuberculosis (M.tb) H37Rv or stimulated with 15 μg/ml Nef or both for 24h. For Panel A, cells were stained with Annexin V-APC. Thin lines represent stimulation with Pam3CSK4 (Pam) alone and the thick lines represent stimulations as indicated. Bar graphs show relative MFIs of the histograms. Data from one of three independent experiments are shown. For Panel B, PMA stimulated THP1 cells were stimulated as indicated for 24h and cytoplasmic extracts were probed for indicated molecules and analyzed by western blots. Numbers below the blots indicate the relative intensities of the bands. Data from one of three experiments are shown. In Panel A, P<0.02 for Pam vs Pam+M.tb. P<0.02 for Pam vs Pam+Nef, P<0.006 for Pam vs Pam+M.tb+Nef.