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. 2015 Jul 1;10(7):e0131767. doi: 10.1371/journal.pone.0131767

Fig 8. Rv3416 synergizes with Nef sufficient HIV but not Nef deficient HIV to inhibit apoptosis in macrophages.

Fig 8

PMA stimulated THP1 cells were stimulated with 1 μg/ml Pam3CSK4 (Pam) and infected with 0.5 MOI of wild type HIV or Nef deficient HIV (HIVδNef) or Vpu deficient HIV (HIVδVpu) in the presence or absence of 20 μg/ml Rv3416 for 48h. Cells were stained with Annexin V-APC. Thin lines represent stimulation with Pam3CSK4 alone and the thick lines represent stimulation as indicated. Data from one of three independent experiments are shown. Bar graphs show relative MFIs of the histograms. Data from one of three experiments are shown. P<0.03 for Pam vs Pam+Rv3416. P<0.04 for Pam vs Pam+HIV, P<0.03for Pam vs Pam+Rv3416+HIV.