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. Author manuscript; available in PMC: 2016 Jul 28.
Published in final edited form as: J Control Release. 2015 May 21;210:208–216. doi: 10.1016/j.jconrel.2015.05.278

Fig. 6. M2e-specific IgG antibodies and inflammatory IL-6 cytokine in bronchoalveolar lavage fluids and viral titers in lungs.

Fig. 6

Levels of IgG antibodies, inflammatory IL-6 cytokine, and viral lung titers were determined at day 4 post-challenge with 4× LD50 of A/Philippines (H3N2) virus from mice (N=4) that were immunized with M2e5x VLP via MN skin or IM route. (A) M2e specific IgG antibody response. IgG antibody responses were determined by ELISA using human M2e peptide as a coating antigen (4 μg/ml). (B) Inflammatory IL-6 cytokine in bronchoalveolar lavage fluids (BALF). IL-6 cytokine was determined by a commercial cytokine ELISA IL-6 kit. (C) Lung viral titers. Lung viral titers were determined by an egg inoculation assay. Asterisks indicate significant differences (*: <0.05, **: <0.01). Data represent mean ± SEM. BALF, bronchoalveolar lavage fluid; EID, egg infectious dose; IL, interleukin; pg, picogram.