Fig. 3.

Labeling of the subsarcolemmal cytoskeleton after injury. Tissue sections from injured TAs were labeled for dystrophin (AC), β-spectrin (D–F), and desmin (G–I). The left column shows images of the respective molecules of interest. EBD-positive fibers from the same section are shown in the middle column, and the right column shows the overlay of the EDB (red) with fluorescent labeling (green). EBD was administered by intraperitoneal injection 24 h before injury. Fibers that were permeable to EBD also lacked dystrophin (arrows, A–C). β-Spectrin and desmin were much less affected in injured fibers (arrows, D–F and G–I, respectively). Scale bar = 50 μm.