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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Am J Physiol Cell Physiol. 2003 Oct 1;286(2):C230–C238. doi: 10.1152/ajpcell.00199.2003

Fig. 4.

Fig. 4

Double labeling of dystrophin and desmin. Fluorescent confocal images (×25) from double labeling of dystrophin and desmin of uninjured (A–C) and injured (D–F) tissue sections. Sections were labeled with monoclonal antibodies against dystrophin (B and E and light blue in overlay C or yellow/green in overlay F) and polyclonal antibodies against desmin (A and D and blue in C and F). In noninjured muscle, desmin labeling is throughout the fibers and dystrophin labeling is circumferential as expected. In injured fibers, loss of desmin labeling is more diffuse (asterisk in D), and there is incomplete labeling of dystrophin (arrow in E). Scale bar = 25 μm.