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. 2015 Jul 2;11(7):e1005008. doi: 10.1371/journal.ppat.1005008

Fig 3. Epithelial B4galnt2-expression increases invasion by S. Typhimurium.

Fig 3

(A) Carnoy’s fixed cecal sections were stained by FISH (Gam42a probe) to visualize bacteria. Measurement of Salmonella adherence (t 2.286 = -1.349, P = 0.2954, unpaired t-test) and mucosa invasion (t 2.430 = -3.681, P = 0.0491; bacterial counts in 10 high power fields per individual, N = 3; unpaired t-test). (B) B4galnt2 expression in Mode-K cells after transfection with B4galnt2 specific siRNA and scrambled siRNA relative to untreated cells (t 3.025 = -3.3601, P = 0.0432; unpaired t-test). (C) Salmonella invasion of Mode-K cell cultures transfected with B4galnt2 specific and scrambled siRNA, infected with S. Typhimurium. There is no significant effect of B4galnt2 expression for adhesion of the bacteria to Mode-K cells (0.5 h: F 1,14 = 3.133, P = 0.0985; LMM with experiment as random factor), while invasion of S. Typhimurium into Mode-K cells expressing B4galnt2 was slightly better than into cells with B4galnt2 knockdown (1 h: F 1,14 = 7.644, P = 0.0152, 4 h: F 1,14 = 26.336, P = 0.0002; LMM with experiment as random factor; # P < 0.100, * P < 0.050, ** P < 0.010, *** P < 0.001; error bars indicate SEM).