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. 2015 Jun 29;7:19–24. doi: 10.4137/BIC.S19079

Figure 1.

Figure 1

RT-PCR results from breast cancer samples, testis tissue, and breast cancer cell lines. (A) RT-PCR was performed to analyze of WBP2NL gene expression in breast cancer samples, testis tissue, and cell lines. Lane 1 and 2 are cDNA from MCF-7 and MDA-MB-231 cell line, respectively. Lane 3 is cDNA from an adult human testis as a positive control. Lanes 4–12 are cDNAs from breast cancer samples, Lane 13 is the negative control (water), and Lane 14 is 100 bp DNA marker. (B) Nested PCR was performed to analyze low expression of WBP2NL gene in breast cancer and cell lines. (C) All samples were controlled for presence of cDNA using the housekeeping gene HPRT.