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. 2015 Jul 2;10(7):e0127670. doi: 10.1371/journal.pone.0127670

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© 2015 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — The arrow heads indicate that HIBADH protein was mainly localized at sperm neck-piece and mid-piece, and to a lesser extent, in the sperm head. (A) Negative control. (B) Nuclear DNA signals (blue). (C) HIBADH protein signals (green). (D) Merging of HIBADH protein (green) and nuclear DNA signals (blue). Image was obtained using an inverted microscope (OLYMPUS) at 40 × 10. Images were obtained on a 5 μm scale plate.