Table 2. Primer information used for cloning the bovine HIBADH gene promoter region.
| Primer names | Sequences (5′-3′) | Annealing temperature (℃) | The size of production (bp) |
|---|---|---|---|
| Q-1 | F:GCTACCATGCCAAGAACCCT | 62.5 | 1430 |
| R:TGCTAGGAACTTGCTCCTCAA | |||
| Q-2 | F:ACACACAAACATACTTTCTGCACT | 59.7 | 1675 |
| R: GGCCCTCCAATTGTCCTCAA | |||
| Q-3 | F:TGTTTTGGGTGTCCATGGCT | 61.1 | 1812 |
| R:GGCCCTCCAATTGTCCTCAA | |||
| P-1 | F:CGGGGTACCGTCAAAGAAAGGAACTGCT | 61.9 | 2340 |
| R:CCGCTCGAGAATGATACGGAAGGTGGAA | |||
| P-2 | F:CGGGGTACCAGTCAGCTTCTCGAACCAGA | 61.6 | 1805 |
| R:CCGCTCGAGAATGATACGGAAGGTGGAA | |||
| P-3 | F:CGGGGTACCAGAATCTATTCGGCAAATTA | 63.2 | 1495 |
| R:CCGCTCGAGAATGATACGGAAGGTGGAA | |||
| P-4 | F:CGGGGTACCAGGCGCTGCGATTAGA | 60.1 | 618 |
| R:CCGCTCGAGAATGATACGGAAGGTGGAA | |||
| G | F:CTCCAACATCACAGTTCAAAAG | 62.5 | 967 |
| R:TACCCGCTGCAAGGCT | |||
| M-outer | F:ATTTGGGATTTTTGATTGATTTTT | 52.2 | 218 |
| R:CTCACCCACTAACTAACAAAATAC | |||
| M-inner | F:GGGGTTTGTAAGGTGAT | 52.2 | 175 |
| R:GGCGGTAACAAAATACGTAATA | |||
| PT | F:GCATGGCAGCCTCCTTACG | 61.2 | 1254 |
| R:AGAGACCATCAGTGGCTTGC |
Note: The Kpn I sites (CGGGGTACC) were added to the forward primers (P-1, P-2, P-3, and P-4) as recognition and protection sites. The reverse primers (P-1, P-2, P-3, and P-4) (3′) contained the Xho I sites (CCGCTCGAG).