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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Hum Mutat. 2014 Jun 24;35(8):998–1010. doi: 10.1002/humu.22593

Figure 6.

Figure 6

Cohesin/HP1γ binding, H3K9me3, and DNA methylation are intact on non-4q/10q D4Z4 homologs in FSHD1 and FSHD2 cells. A: ChIP-qPCR analysis using antibodies specific for H3K9me3, H3K27me3, and methylcytidine (metC) using 4q/10q-specific (4q/10q-Q-PCR) and non-4q/10q-specific (Q-PCR-F + chr 3-R, chr 15-F + 4q-Hox-R, Q-PCR-F + chr 22-R) PCR primers as indicated at the top. Four normal control (white bars), six FSHD1 (grey bars), and one FSHD2 (black bar) myoblast samples were analyzed. The quantitative real-time PCR was normalized with input DNA and preimmune controls as indicated. P-values for significant differences between normal and FSHD samples are indicated. B: ChIP-qPCR analysis of cohesin and HP1γ in control and FSHD1 myoblasts. C: ChIP-qPCR analysis of normal control and FSHD2 fibroblasts using antibodies specific for H3K9me3, H3K27me3, cohesin, HP1γ, and met-C as indicated.