Figure 7.
Sequencing analysis of non-4q/10q D4Z4 homologs in human cells. H3K9me3 ChIP and genomic DNA from the indicated human cell samples were used for PCR analysis using 4q/10q-specific (Q-PCR-F + Q-PCR-R) and non-4q/10q homolog-specific (Q-PCR-F + chr 3-R, chr 15-F + 4q-Hox-R, Q-PCR-F + chr 22-R) primers. Sequencing results using Q-PCR and 4q-Hox-R as used in Figure 3A are shown at the top. Nucleotide numbers are indicated. A heat map of the nucleotide variation was made similar to Figure 3A. Gray indicates no change, whereas red and green indicate a shift to A/T and C/G, respectively. Black indicates insertion and deletion (indel). The results are also summarized in the Supp. Table S3. The bracket for each cell line is proportional to the number of clones analyzed.