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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: J Pharmacol Sci. 2015 Jan 5;127(1):10–16. doi: 10.1016/j.jphs.2014.12.013

Fig. 2.

Fig. 2

The sigma-1 receptor (green signal in the left and middle panels) and the DMT producing enzyme INMT (green in the right panel) are localized to postsynaptic sites of C-terminals and juxtaposed to presynaptic cholinergic (ChAT positive) boutons (red). To demonstrate that the sigma-1 receptor is juxtaposed only to cholinergic postsynaptic densities of MNs we performed double labeling with antibodies against synaptophysin (a universal marker for different types of chemical synapses). Notice that not all synaptophysin-positive synapses are juxtaposed to the sigma-1 receptor. Blue (DAPI stain) indicates cell nuclei. Lower panel shows localization of sigma-1 receptor in subsurface cistern of cholinergic postsynaptic site. Modified with copyright permission from: Mavlyutov et al., Neuroscience 2012; 206: 60–80.