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MDM were incubated for 1hr with 20μM of MAPK signalling inhibitors for p38 (SB202190), JNK (SP600125) and ERK1/2 (U0126) pathways, prior to addition of 4mM PBA (blue), 100nM 25(OH)D3 (red) or co-treatment (purple) and gene expression analysed 24 hrs post-treatment. Mean ± SD, n = 3–4; 2-way ANOVA with Dunnett’s multiple comparisons test: *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.
