Table 1. Effect of 4mM PBA on MDM gene expression and protein secretion.
| Mtb-infected | Uninfected | ||||||
|---|---|---|---|---|---|---|---|
| 24 hrs | 72 hrs | 72 hrs 1 | |||||
| Analyte | Fold change 2 | P | Fold change | P | Fold change | P | |
| mRNA | PROC | 15.31 | 0.0015 | 20.14 | 0.0096 | 23.33 | 0.0017 |
| LTF | 6.78 | 0.0079 | 6.23 | 0.0225 | - | ns | |
| IL36G | 5.52 | 0.0213 | 6.62 | 0.0229 | - | ns | |
| IL1B | 4.29 | 0.0116 | 3.82 | 0.0252 | 6.56 | 0.0036 | |
| IL37 | 3.80 | 0.0028 | 5.26 | 0.0099 | - | ns | |
| IL23A | 2.84 | 0.0094 | - | ns | - | ns | |
| CXCL8 | 1.92 | 0.0461 | 3.24 | 0.0090 | 6.92 | 0.0045 | |
| TREM1 | 1.85 | 0.0453 | 2.82 | 0.0427 | - | ns | |
| CASP1 | 1.81 | 0.0001 | 2.12 | 0.0487 | - | ns | |
| NCF4 | 1.53 | 0.0042 | - | ns | - | ns | |
| ARG1 | -1.45 | 0.0085 | - | ns | - | ns | |
| CYP27B1 | -1.61 | 0.0347 | - | ns | -6.25 | 0.0005 | |
| IL10 | -2.74 | 0.0208 | - | ns | - | ns | |
| CXCL7 | -4.03 | 0.0104 | - | ns | - | ns | |
| IL12A | - | ns | 6.21 | 0.0093 | 2.35 | 0.0067 | |
| CAMP | - | ns | 3.40 | 0.0030 | 3.90 | 0.0189 | |
| NLRP3 | - | ns | 2.44 | 0.0263 | - | ns | |
| VDR | - | ns | 2.14 | 0.0121 | 2.42 | 0.0096 | |
| IFNB1 | - | ns | - | ns | 1.72 | 0.0168 | |
| Protein | CCL3 | - | ns | 19.73 | 0.0032 | ||
| CCL5 | - | ns | 6.91 | 0.0023 | |||
| IL-2R | - | ns | 5.99 | 0.0018 | |||
| CCL4 | - | ns | 3.16 | 0.0363 | |||
| CCL2 | -10.54 | 9.17x10-5 | -5.78 | 0.0269 | |||
1Uninfected mRNA had no genes significant affected by PBA at 24 hrs (q≤0.1).
2Fold change and P-value from t-test for GLM with adjustment for subject ID (repeated measures, n = 5), on samples from the specified time point and infection status, non-significant (ns) if false discovery rate (q-value) >0.1