Table 2. Effect of 25(OH)D and PBA co-treatment on Mtb-infected MDM gene expression and protein secretion.
| 25(OH)D vs Control | 25(OH)D+PBA vs Control | 25(OH)D+PBA vs PBA | |||||
|---|---|---|---|---|---|---|---|
| Analyte | Fold change 1 | P | Fold change 1 | P | Fold change 1 | P | |
| mRNA | CYP24A1 | 2922.85 | 2.29 x10-7 | 9740.72 | 6.29 x10-10 | 4260.58 | 5.94 x10-9 |
| CAMP | 54.01 | 0.0002 | 195.63 | 0.0003 | 105.96 | 9.70 x10-5 | |
| IL37 | 12.6 | 2.41 x10-5 | 35.91 | 1.22 x10-6 | 7.25 | 5.00 x10-5 | |
| IL36G | 8.85 | 0.0014 | 14.4 | 0.0009 | 2.84 | 0.0222 | |
| TREM1 | 5.27 | 8.54 x10-5 | 6.92 | 1.26 x10-5 | 3.98 | 0.0002 | |
| NOD2 | 2.45 | 0.0049 | - | ns | - | ns | |
| PROC | - | ns | 9.09 | 1.24 x10-5 | -3.74 | 0.0067 | |
| ARG1 | - | ns | 3.62 | 0.0384 | 2.22 | 0.0342 | |
| LTF | - | ns | 3.45 | 0.0075 | - | ns | |
| IL1B | - | ns | 3.19 | 0.0395 | - | ns | |
| IL12A | - | ns | - | ns | -3.86 | 0.0109 | |
| IL10 | - | ns | -1.56 | 0.0372 | - | ns | |
| IFNG | - | ns | -3.24 | 0.0563 | - | ns | |
| VDR | -2.09 | 0.0036 | -1.70 | 0.0152 | -2.35 | 0.0011 | |
| TNF | -2.56 | 0.0136 | -2.04 | 0.0571 | - | ns | |
| CXCL9 | -4.16 | 0.0011 | -8.94 | 0.0394 | - | ns | |
| CYP27B1 | -6.15 | 0.0033 | -3.17 | 0.001 | -2.56 | 0.0022 | |
| CASP1 | -5.78 | 1.70 x10-5 | - | ns | -3.00 | 0.001 | |
| CXCL7 | -8.76 | 0.0015 | -25.67 | 0.0001 | -3.96 | 0.0062 | |
| CXCL10 | -9.54 | 0.0031 | -33.02 | 0.0027 | -10.67 | 0.0162 | |
| Protein | IL-1B | 2.84 | 0.0012 | - | ns | - | ns |
| CXCL9 | - | ns | -2.31 | 0.0299 | - | ns | |
| CXCL10 | -1.69 | 0.0172 | -2.43 | 0.0027 | -2.63 | 0.0045 | |
| CCL2 | -5.00 | 0.0004 | -23.13 | 0.0001 | -2.77 | 0.0156 | |
| CCL5 | - | ns | - | ns | -2.21 | 0.0157 | |
1Fold change and p-value from t-test for GLM on indicated samples at 24 and 72 hrs with adjustment for subject ID (repeated measures, n = 3–4) and time; non-significant (ns) if false discovery rate (q-value) >0.1