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. 2015 Jul 2;10(7):e0131428. doi: 10.1371/journal.pone.0131428

Fig 4. Effect of EDCs alone and in combination on the secretion of IL-8 by differentiated THP-1 cells.

Fig 4

Differentiated THP-1 cells were exposed to different concentrations of BPA, DEHP, DBP, 4-OP or a combination of both for 24 hours. Culture medium was replaced by fresh medium containing 10 ng/mL of LPS for a subsequent 24 hours of incubation. IL-8 concentrations were determined using a sandwich ELISA test. Values are means ± SD (N = 3). Each experiment has been performed three times and in triplicate. a: vs control (Tukey’s test, p<0.05); b: vs combination (One-way ANOVA, Tukey post test, p<0.05).