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. 2015 Jul 1;23(4):345–349. doi: 10.4062/biomolther.2015.019

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Copyright ©2015, The Korean Society of Applied Pharmacology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Antiviral activity BeA against influenza A/PR/8 virus in A549 cells. Antiviral activity of BeA was assessed against influenza A/PR/8 virus in A549 cells. The indicated concentrations of BeA was determined using a concentration series of 0.4, 2, 10, and 50 μM. After 48 h of incubation, the antiviral activity was investigated by CPE reduction assay using SRB. (A) The structure of betulinic acid. (B) Absorbance in each well was measured using microplate reader. ^***p<0.0001 and n.s. for not significant. (C) The percent value was calculated by absorbance value as mentioned in Materials and Methods. Results are presented as the mean ± SD of the absorbance and percentage values obtained from three independent experiments carried out in triplicate. ^*p<0.001; ^**p<0.03; ^***p<0.0001 and n.s. for not significant.