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. 2015 Jul 1;23(4):357–366. doi: 10.4062/biomolther.2015.005

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Copyright ©2015, The Korean Society of Applied Pharmacology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Isorhamnetin protects HaCaT keratinocytes against UVB-induced oxidative macromolecular damage. (A) DNA damage was detected by the comet assay. (B and C) Lipid peroxidation was detected by (B) measurement of 8-isoprostane levels in the conditioned media and (C) detection of lipid hydroperoxides in DPPP-stained cells by fluorescence microscopy. (D) Protein oxidation was assayed by measurement of protein carbonyl levels. ^*Significantly different from control (p<0.05); ^#significantly different from UVB-irradiated cells (p<0.05). Combination, isorhamnetin+UVB light.