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. 2015 Jul 2;10(7):e0131244. doi: 10.1371/journal.pone.0131244

Table 1. List of primers used in RT-PCR, RACE-PCR, and real time PCR.

Gene Primer Name Function Primer sequence
Hsp70 Hsp70-F Fragment cloning GARATHATHGCNAAYGA
Hsp70-R Fragment cloning ACRTCRAANGTNCCNCC
Hsp70-5′ 5′-RACE PCR GTAATAACTGCCTCACGAACCTGCCCAC
Hsp70-3′ 3′-RACE PCR GGTGCTGCGGATTATCAACGAGCCAACT
Hsp70-FC-F Full length cloning CAGAAGAAAATGCCGAAGATTCC
Hsp70-FC-R Full length cloning TTAACAGTTCACTTAGTCAACCTCCTC
Hsp70-RT-F Real time PCR AGCACTGACCGATGCCAAGAT
Hsp70-RT-R Real time PCR GCACTGACCGATGCCAAGAT
Grp78 Grp78-F Fragment cloning GARATHATHGCNAAYGA
Grp78-R Fragment cloning ACRTCRAANGTNCCNCC
Grp78-5′ 5′-RACE PCR ATACGACGGCGTGATTCTGTTTCCTTG
Grp78-3′ 3′-RACE PCR CCTACGGACTGGACAAGAAAGAGGGAGA
Grp78-FC-F Full length cloning ATAAACCCACCATGAGGGGAATTC
Grp78-FC-R Full length cloning GACCGACTTTTACAGTTCATCCTT
Grp78-RT-F Real time PCR CCAAAGGTGCAGCAACTTGTAAA
Grp78-RT-R Real time PCR CAATCGCATCTGTATCCTGTTCT
Hsp40 Hsp40-F Fragment cloning AARTAYCAYCCNGAYAARAAY
Hsp40-R Fragment cloning GCYTT CCA NCCNGG YTT NAC
Hsp40-5′ 5′-RACE PCR GTAAATGGGTCATCCAACTCC
Hsp40-3′ 3′-RACE PCR AGTTGGATGACCCATTTACCT
Hsp40-FC-F Full length cloning ATCGTTGAAGATGGGGAAAG
Hsp40-FC-R Full length cloning CCCTCACAGTAACATTCAAT
Hsp40-RT-F Real time PCR AGTTGGATGACCCATTTACCT
Hsp40-RT-R Real time PCR TTTCTTCGTGCAACCTCTTAG
Hsp90 Hsp90-F Fragment cloning ACNAARGCNTTYATGGARGC
Hsp90-R Fragment cloning TCRCARTTRTCCATDATRAA
Hsp90-5′ 5′-RACE PCR CAGATTGGCTTGGTCTTGTTT
Hsp90-3′ 3′-RACE PCR CCGAAGATTGAAGATGTTGGA
Hsp90-FC-F Full length cloning TGTCCAAGATGCCGGAGGAC
Hsp90-FC-R Full length cloning TTAATCAACTTCCTCCATTCGAGAG
Hsp90-RT-F Real time PCR AAACAAGACCAAGCCAATCTG
Hsp90-RT-R Real time PCR AATACCCTGCGGACATACAAT
β-actin β-actin-RT-F Real time PCR CGAAGCACAGTCAAAGAGAGGTA
β-actin-RT-R Real time PCR GCTTCAGTCAAGAGAACAGGATG