Table 1. List of primers used in RT-PCR, RACE-PCR, and real time PCR.
Gene | Primer Name | Function | Primer sequence |
---|---|---|---|
Hsp70 | Hsp70-F | Fragment cloning | GARATHATHGCNAAYGA |
Hsp70-R | Fragment cloning | ACRTCRAANGTNCCNCC | |
Hsp70-5′ | 5′-RACE PCR | GTAATAACTGCCTCACGAACCTGCCCAC | |
Hsp70-3′ | 3′-RACE PCR | GGTGCTGCGGATTATCAACGAGCCAACT | |
Hsp70-FC-F | Full length cloning | CAGAAGAAAATGCCGAAGATTCC | |
Hsp70-FC-R | Full length cloning | TTAACAGTTCACTTAGTCAACCTCCTC | |
Hsp70-RT-F | Real time PCR | AGCACTGACCGATGCCAAGAT | |
Hsp70-RT-R | Real time PCR | GCACTGACCGATGCCAAGAT | |
Grp78 | Grp78-F | Fragment cloning | GARATHATHGCNAAYGA |
Grp78-R | Fragment cloning | ACRTCRAANGTNCCNCC | |
Grp78-5′ | 5′-RACE PCR | ATACGACGGCGTGATTCTGTTTCCTTG | |
Grp78-3′ | 3′-RACE PCR | CCTACGGACTGGACAAGAAAGAGGGAGA | |
Grp78-FC-F | Full length cloning | ATAAACCCACCATGAGGGGAATTC | |
Grp78-FC-R | Full length cloning | GACCGACTTTTACAGTTCATCCTT | |
Grp78-RT-F | Real time PCR | CCAAAGGTGCAGCAACTTGTAAA | |
Grp78-RT-R | Real time PCR | CAATCGCATCTGTATCCTGTTCT | |
Hsp40 | Hsp40-F | Fragment cloning | AARTAYCAYCCNGAYAARAAY |
Hsp40-R | Fragment cloning | GCYTT CCA NCCNGG YTT NAC | |
Hsp40-5′ | 5′-RACE PCR | GTAAATGGGTCATCCAACTCC | |
Hsp40-3′ | 3′-RACE PCR | AGTTGGATGACCCATTTACCT | |
Hsp40-FC-F | Full length cloning | ATCGTTGAAGATGGGGAAAG | |
Hsp40-FC-R | Full length cloning | CCCTCACAGTAACATTCAAT | |
Hsp40-RT-F | Real time PCR | AGTTGGATGACCCATTTACCT | |
Hsp40-RT-R | Real time PCR | TTTCTTCGTGCAACCTCTTAG | |
Hsp90 | Hsp90-F | Fragment cloning | ACNAARGCNTTYATGGARGC |
Hsp90-R | Fragment cloning | TCRCARTTRTCCATDATRAA | |
Hsp90-5′ | 5′-RACE PCR | CAGATTGGCTTGGTCTTGTTT | |
Hsp90-3′ | 3′-RACE PCR | CCGAAGATTGAAGATGTTGGA | |
Hsp90-FC-F | Full length cloning | TGTCCAAGATGCCGGAGGAC | |
Hsp90-FC-R | Full length cloning | TTAATCAACTTCCTCCATTCGAGAG | |
Hsp90-RT-F | Real time PCR | AAACAAGACCAAGCCAATCTG | |
Hsp90-RT-R | Real time PCR | AATACCCTGCGGACATACAAT | |
β-actin | β-actin-RT-F | Real time PCR | CGAAGCACAGTCAAAGAGAGGTA |
β-actin-RT-R | Real time PCR | GCTTCAGTCAAGAGAACAGGATG |