Skip to main content
. 2015 Jul 2;10(7):e0132075. doi: 10.1371/journal.pone.0132075

Fig 6. Fragmented burst neuron perineuronal net immunostaining.

Fig 6

Parvalbumin-positive (PAV) putative burst neurons in the rostral interstitial nucleus of the medial longitudinal fasciculus (RIMLF) and in the paramedian pontine reticular formation (PPRF) were present in both the control and patient. Perineuronal nets appeared normal around burst neurons (EBN) in the PPRF (A, C, arrows) and RIMLF (F, H, arrows) in the control, here immunostained for aggrecan (ACAN). However, the patient’s burst neurons in the PPRF were ensheathed only by fragments of ACAN-based perineuronal nets (B, D, short arrow). Few PAV-positive neurons were found with preserved perineuronal nets (B, E, long arrow). Similarly, the PAV-positive neurons (brown) in the RIMLF of the control are enwrapped by distinct perineuronal nets (F, H, arrows), whereas only fragments are found in the RIMLF of the patient (G, short arrows). Most PAV-positive neurons are attached to few ACAN-positive fragments (I, short arrow). Scale bars: A,B,F,G = 200μm; C,D,E,H,I = 30μm.