Fig 3. The effect of autophagy inhibition on the cytotoxicity of Huaier extract.

MDA-MB-231 (A and B), MDA-MB-468 (C and D) and MCF7 (E and F) were pretreated with 3-MA or CQ before being exposed to Huaier extract for the indicated time. The cell viability was measured using the MTT assay. The experiments were performed in triplicate and data presented as the mean ± SD of three separate experiments. ^*p < 0.05, ^**p < 0.01.