Fig 6. H₂O₂ and doxorubicin treatments show different alteration on MLC phosphorylation.

Representative images of F-actin staining (red), p-MLC (green), and nucleus (blue) in WT and ROCK1 ^-/- MEFs treated with 200 μM of H₂O₂ for 4 h or with 3 μM of doxorubicin for 8 h. Cells showing enriched cortical p-MLC staining are indicated with white arrows. Bar, 25 μm. Doxorubicin induces an increase in p-MLC translocation to cortical actin cytoskeleton in WT MEFs but to a much smaller extent in ROCK1 ^-/- MEFs. WT and ROCK1 ^-/- MEFs treated with H₂O₂ both show diffused cytoplasmic p-MLC staining.