Fig 1. Expression of EZR, PODXL and CLIC5 in rat livers after carcinogenic treatment.
Male rats used in the MRHM were sacrificed at 9 (9 m), 12 (12 m) and 18 (18 m) months. A) Tumor and preneoplastic lesions were characterized by GGT activity (spotted and solid arrows, respectively). B) Analysis of relative expression of genes by qRT-PCR in rat livers. C) Protein expression in fractions isolated from rat livers was assessed by Western blot analysis, representative image. D) Graphic representation of triplicates of western blot. GADPH was included as a loading control. C9 m, C12 m and C18 m represent controls of each time point; 9 mT, 12 mT and 18 mT represent tumor regions; 9 mN represents nodular areas (preneoplastic lesions); and 9 mNT, 12 mNT and 18 mNT represent non-tumor areas of the liver (n = 4 rats per group). □EZR, PODXL and ■CLIC5. Error bars indicate the standard error of the mean (SEM). *p≤0.05, **p≤0.03 and ***p≤0.002 vs. their respectively untreated control values (ANOVA and Tukey–Kramer test).