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. 2015 Jul 2;10(7):e0131761. doi: 10.1371/journal.pone.0131761

Fig 1. The percentage of Th22 cells in representative patients with ND, CR, relapsed-refractory AML patients or in controls.

Fig 1

Heparinized bone marrow from all subjects was stimulated with phorbol myristate acetate, ionomycin, and monensin for 4 h or BM mononuclear cells were isolated by Ficoll-Hypaque gradient centrifugation and then stained with labeled antibodies, as described in the “Materials and methods” section. A. Lymphocytes were gated by flow cytometry. B. The percentage of CD4+IFN-γ- T cells in AML patients and controls. C, D, E, F. The percentage of Th22 (CD4+IFN-γ-IL-17-IL-22+) cells in AML patients and controls.