Table 1.

Examples of Mtb Proteins Expressed in M. smegmatis Expression Host

				Solubility
ORF	Function/annotation	Molecular weight (kDa)	Theoretical pI	Soluble	Insoluble	Oligomeric state	References
Rv0065	VapC1	14.3	4.8	✓			79
Rv0132c	fHMAD	38.4	6.4	✓		Dimer	63, 80
Rv0407	FGD1	36.9	5.1	✓		Dimer	72
Rv0413	MutT3	23.4	4.8		✓		4
Rv0475	HBHA	21.5	9.8	✓			59
Rv0542c	MenE	36.5	5.6		✓		n/a
Rv0617	VapC29	13.9	5.7	✓			79
Rv0901	CP	18.9	10.1				81
Rv0993	GalU	32.3	4.6	✓			82
Rv1096	Possible glycosyl transferase	31	7	✓			83
Rv1160	MutT2	15.1	6.1	✓			4
Rv1186c	PruC	57.5	5.9	✓a			84
Rv1187	PruA	58.8	5.7	✓		Dimer	73
Rv1188	PruB	36.2	8.3	✓a			73
Rv1212c	GlgA	41.5	6.3	✓			82
Rv1213	GlgC	43.8	4.9	✓			82
Rv1601	HisB	22.7	6.8	✓		Monomer	85
Rv1665	PKS11	37.6	5.6	✓		Dimer	71
Rv1886c	FbpB	34.5	5.7	✓		Monomer	86
Rv1980c	MPT64	24.8	4.6	✓			41
Rv1991c	MazF6	12.2	9.9	✓			44
Rv2141c	DapE2	48.1	4.7	✓			4
Rv2220	GlnA1	53.5	4.8	✓		Dodecamer	87–88
Rv2245	KasA	43.2	4.9	✓		Dimer	89
Rv2420c	RsfS	13.8	4.5	✓		Dimer	PDB code 4WCW
Rv2611c	Possible acetyltransferase	35	9.1	✓			4
Rv2903c	LepB	31.8	5.7		✓		n/a
Rv2924c	MutM	31.9	10.3	✓			4
Rv3127	CP	38.4	7.6		✓		4
Rv3177	Possible peroxidase	30.8	8.9		✓		4
Rv3248c	SahH	54.3	4.8	✓			90
Rv3490	OtsA	55.8	6.7	✓			82
Rv3547	Ddn	17.3	9.9	✓a			4
Rv3565	AspB	41	4.6	✓			91
Rv3713	CobQ2	24.1	6.1	✓			4
Rv3772	HisC2	38	6.5	✓			92
Rv3855	EthR	23.7	4.9	✓		Dimer	93
Rv3874	CFP-10	10.8	4.3	✓		Dimer	94

^aSoluble aggregates.

CP, conserved protein.