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. Author manuscript; available in PMC: 2016 Jun 30.
Published in final edited form as: Cell Rep. 2015 Jun 18;11(12):1885–1891. doi: 10.1016/j.celrep.2015.05.043

Figure 3. Recruitment of eIF2α is mediated by the GADD34 PEST domain.

Figure 3

(a) Immuno-precipitation (IP) experiment using WT- and KARA-GADD34. GADD34 recruits eIF2α from the HEK293T cell lysate independent of PP1 binding. (b) IP experiment using His6-GADD34241-674 and His6-GADD34513-631. Only His6-GADD34241-674 binds eIF2α (HEK293T cells lysate), while both His6-GADD34241-674 and GADD34513-631 bind PP1. (c) FLAG vector or FLAG-GADD34 proteins were expressed in HEK293 cells and IP using anti-FLAG-conjugated to agarose beads. The IP and whole cell lysates (WCL) were subjected to immunoblotting (IB) with indicated antibodies. Molecular weight markers (kD) are shown.