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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biomaterials. 2015 Jun 3;63:24–34. doi: 10.1016/j.biomaterials.2015.06.002

Fig. 3.

Fig. 3

Organoid stiffness affects 40LB stromal network and CD40L expression. (A) Fluorescence images of the 40LB stromal cell network formation with concentrations of gelatin and SiNP at 40,000 cells. Scale bar 200 µm (B) Fluorescence images of the 40LB stromal cell network formation with 2% gelatin and 1.5% SiNP and varying 40LB seeding density. Scale bar 200 mm (C) Histogram representing the overlay of CD40L expression for 2D culture of 40LBs on tissue culture plates versus 3D encapsulation in 2% gelatin with 1.5% SiNP organoids (Left). Bar graph representing mean fluorescence intensity of CD40L ligand expression in 2D and 3D cultures of 40LB (Right, Mean ± S.E.M, n = 4). *P < 0.05, 2-way ANOVA with post-hoc Bonferroni correction.