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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Clin Cancer Res. 2015 Feb 17;21(13):2952–2962. doi: 10.1158/1078-0432.CCR-14-2998

Figure 5. Activation of the iC9 suicide gene eliminates engrafted K562-derived whole-cell vaccine in vivo.

Figure 5

Panel A. Characterization of the clones by flow cytometry analysis. Gray areas indicate wild type K562 cells. Panel B. Western blot showing the expression of CMV-pp65 in the clones expressing the iC9 transgene. Panels C and D. NOG/SCID/γc−/− mice were inoculated subcutaneously with irradiated (C) or non-irradiated (D) K562-derived whole-cell vaccine expressing the iC9 gene and labeled with an enhanced firefly luciferase. Tumor growth was measured by in vivo imaging. Panel E. Effects of the administration of the chemical inducer of dimerization (CID) AP20187 on the growth of engineered vaccine.